The antibody detects endogenous level of total GFAP protein.
Purification
Affinity purified
Immunogen
Peptide sequence around AA 423-427 (E-S-K-Q-E) derived from Human GFAP. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
GFAP
Reactivity: Rat
WB, IHC, ICC, IP
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Western blotting: 1:500-1:1000 Immunofluorescence: 1:100-1:200
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Target
GFAP
(Glial Fibrillary Acidic Protein (GFAP))
Alternative Name
GFAP
Background
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.